Rabbit anti-Human Dkk-1 Polyclonal Antibody | anti-DKK1 antibody

Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)

Cat. Num. AAA611995

• Gene Names: DKK1; SK; DKK-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by peptide affinity chromatography.
• Synonyms: Dkk-1; Polyclonal Antibody; Dkk-1 (DKK1; SK; DKK-1; Dickkopf Homolog 1 (Xenopus laevis); Dickkopf-1 Like; Dickkopf (Xenopus laevis) Homolog 1); Anti -Dkk-1 (DKK1; anti-DKK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects transfected levels of DKK1 protein. This antibody does not cross-react with DKK2.
• Purity/Purification: Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-DKK1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Gln75 of human DKK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing DKK1, using MBS611995 DKK1.)

Related Product Information for anti-DKK1 antibody

Dickkopf (DKK) family of proteins consist of four members DKK1, DKK2, DKK3 and DKK4 and function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14).

Product Categories/Family for anti-DKK1 antibody

Antibodies; Abs to Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374.1
• NCBI GenBank Nucleotide #: NM_012242.2
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28,672 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf homolog 1 (Xenopus laevis)
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1; hDkk-1; dickkopf-1 like; OTTHUMP00000019617; dickkopf related protein-1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK1
• UniProt Entry Name: DKK1_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq]

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; early endosome membrane; extracellular region; plasma membrane

Molecular Function: signal transducer activity; protein binding; receptor antagonist activity; growth factor activity; low-density lipoprotein receptor binding

Biological Process: negative regulation of skeletal muscle development; cellular morphogenesis during differentiation; negative regulation of Wnt receptor signaling pathway; response to retinoic acid; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; hair follicle development; negative regulation of mesodermal cell fate specification; mesoderm formation; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis