Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Mouse phospholipase A2, group V ELISA Kit | PLA2G5 elisa kit

Mouse Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit

Gene Names
Pla2g5; PLA2; sPLA2
Reactivity
Mouse
Synonyms
phospholipase A2; group V; Mouse Calcium-dependent phospholipase A2; PLA2G5 ELISA Kit; Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA kit; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2); Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase; PLA2G5 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G5. No significant cross-reactivity or interference between Mouse PLA2G5 and analogues was observed.
Sequence Length
137
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Lipogenesis and lipometabolism
Protein Biological Process 3
Lipid degradation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,859 Da
NCBI Official Full Name
calcium-dependent phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group V
NCBI Official Symbol
Pla2g5
NCBI Official Synonym Symbols
PLA2; sPLA2
NCBI Protein Information
calcium-dependent phospholipase A2; PLA2-10; group V phospholipase A2; phosphatidylcholine 2-acylhydrolase 5
UniProt Protein Name
Calcium-dependent phospholipase A2
UniProt Gene Name
Pla2g5
UniProt Entry Name
PA2G5_MOUSE

Uniprot Description

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family.

Protein type: Phospholipase; Cell surface; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid

Cellular Component: Golgi apparatus; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane

Molecular Function: heparin binding; phospholipase A2 activity; hydrolase activity; calcium-dependent phospholipase A2 activity; metal ion binding; calcium ion binding; receptor binding

Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; phospholipid metabolic process; lipid metabolic process; arachidonic acid secretion; lipid catabolic process

Research Articles on PLA2G5

Similar Products

Product Notes

The Mouse PLA2G5 pla2g5 (Catalog #AAA941705) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA941705 ELISA Kit recognizes Mouse PLA2G5. It is sometimes possible for the material contained within the vial of "phospholipase A2, group V, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.