Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Calcium-dependent phospholipase A2 (PLA2G5) Standard Curve.)
Mouse Calcium-Dependent Phospholipase A2 (PLA2G5) ELISA Kit | PLA2G5 elisa kit
Mouse Calcium-Dependent Phospholipase A2 (PLA2G5) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Calcium-dependent phospholipase A2 (PLA2G5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Calcium-dependent phospholipase A2 (PLA2G5) Standard Curve.)
Background: PLA2G5 is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol.
NCBI and Uniprot Product Information
Uniprot Description
PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family.
Protein type: Phospholipase; Cell surface; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid
Cellular Component: Golgi apparatus; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane
Molecular Function: heparin binding; phospholipase A2 activity; hydrolase activity; calcium-dependent phospholipase A2 activity; metal ion binding; calcium ion binding; receptor binding
Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; phospholipid metabolic process; lipid metabolic process; arachidonic acid secretion; lipid catabolic process
Research Articles on PLA2G5
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Product Notes
The Mouse PLA2G5 pla2g5 (Catalog #AAA9717347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717347 ELISA Kit recognizes Mouse PLA2G5. It is sometimes possible for the material contained within the vial of "Calcium-Dependent Phospholipase A2 (PLA2G5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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