Typical Testing Data/Standard Curve (for reference only)
Mouse phospholipase A2, group XIIA ELISA Kit | PLA2G12A elisa kit
Mouse Group XIIA secretory phospholipase A2, PLA2G12A ELISA Kit
Gene Names
Pla2g12a; GXII; Rossy; GXII-1; Pla2g12; mGXII-1; GXII-1-PLA2; 2310004B05Rik
Reactivity
Mouse
Synonyms
phospholipase A2; group XIIA; Mouse Group XIIA secretory phospholipase A2; PLA2G12A ELISA Kit; Mouse Group XIIA secretory phospholipase A2 (PLA2G12A) ELISA kit; GXII; PLA2G12; ROSSY; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; PLA2G12A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse PLA2G12A. No significant cross-reactivity or interference between mouse PLA2G12A and analogues was observed.
Sequence Length
192
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G12A elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G12A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G12A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,319 Da
NCBI Official Full Name
group XIIA secretory phospholipase A2 isoform 1
NCBI Official Synonym Full Names
phospholipase A2, group XIIA
NCBI Official Symbol
Pla2g12a
NCBI Official Synonym Symbols
GXII; Rossy; GXII-1; Pla2g12; mGXII-1; GXII-1-PLA2; 2310004B05Rik
NCBI Protein Information
group XIIA secretory phospholipase A2; group XII-1 phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A
UniProt Protein Name
Group XIIA secretory phospholipase A2
Protein Family
UniProt Gene Name
Pla2g12a
UniProt Synonym Gene Names
Pla2g12; GXII sPLA2; sPLA2-XII
UniProt Entry Name
PG12A_MOUSE
Uniprot Description
PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family.
Protein type: EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Phospholipase; Endoplasmic reticulum
Cellular Component: Golgi apparatus; endoplasmic reticulum; cytoplasm; extracellular region; intracellular
Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding
Biological Process: phospholipid metabolic process; lipid metabolic process; lipid catabolic process
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Product Notes
The Mouse PLA2G12A pla2g12a (Catalog #AAA934962) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA934962 ELISA Kit recognizes Mouse PLA2G12A. It is sometimes possible for the material contained within the vial of "phospholipase A2, group XIIA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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