Human fatty acid synthase ELISA Kit | FASN elisa kit

Human Fatty acid synthase, FASN ELISA Kit

Cat. Num. AAA919053

• Gene Names: FASN; FAS; OA-519; SDR27X1
• Reactivity: Human
• Synonyms: fatty acid synthase; Human Fatty acid synthase; FASN ELISA Kit; Human Fatty acid synthase (FASN) ELISA kit; FAS; MGC14367; MGC15706; OA-519; SDR27X1; short chain dehydrogenase/reductase family 27X; member 1; FASN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human FASN. No significant cross-reactivity or interference between Human FASN and analogues was observed.
• Sequence Length: 2511

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 2: Lipogenesis and lipometabolism
• Protein Biological Process 3: Fatty acid biosynthesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FASN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FASN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FASN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FASN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FASN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 41872631
• NCBI GeneID: 2194
• NCBI Accession #: NP_004095.4
• NCBI GenBank Nucleotide #: NM_004104.4
• UniProt Accession #: P49327; Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
• Molecular Weight: 273,427 Da
• NCBI Official Full Name: fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Official Synonym Symbols: FAS; OA-519; SDR27X1
• NCBI Protein Information: fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1
• UniProt Protein Name: Fatty acid synthase
• Protein Family: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS
• UniProt Entry Name: FAS_HUMAN

NCBI Description

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Uniprot Description

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: Lyase; EC 1.1.1.100; EC 2.3.1.38; EC 2.3.1.41; EC 1.3.1.39; EC 2.3.1.85; EC 2.3.1.39; EC 3.1.2.14; EC 4.2.1.59; Lipid Metabolism - fatty acid biosynthesis; Acetyltransferase; Oxidoreductase; Hydrolase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; glycogen granule; membrane; mitochondrion; cytoplasm; plasma membrane; melanosome; cytosol

Molecular Function: protein homodimerization activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; [acyl-carrier-protein] S-acetyltransferase activity; drug binding; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity

Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process