Rat Prokineticin Receptor 1 ELISA Kit | PKR1 elisa kit

Rat Prokineticin Receptor 1 ELISA Kit

Cat. Num. AAA732329

• Gene Names: PROKR1; ZAQ; PKR1; GPR73; GPR73a
• Reactivity: Rat
• Synonyms: Prokineticin Receptor 1; Rat Prokineticin Receptor 1 ELISA Kit; PKR1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10 ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This PKR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PKR1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKR1 elisa kit

Principle of the Assay: PKR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PKR1. Standards or samples are then added to the microtiter plate wells and PKR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PKR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PKR1 are added to each well to "sandwich" the PKR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PKR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKR1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20373179
• NCBI GeneID: 10887
• NCBI Accession #: NP_620414.1
• NCBI GenBank Nucleotide #: NM_138964.2
• Molecular Weight: 44,770 Da
• NCBI Official Full Name: prokineticin receptor 1
• NCBI Official Synonym Full Names: prokineticin receptor 1
• NCBI Official Symbol: PROKR1
• NCBI Official Synonym Symbols: ZAQ; PKR1; GPR73; GPR73a
• NCBI Protein Information: prokineticin receptor 1; PK-R1; G protein-coupled receptor 73; G-protein coupled receptor 73; G protein-coupled receptor ZAQ; G-protein coupled receptor ZAQ
• UniProt Protein Name: Prokineticin receptor 1
• Protein Family: Prokineticin receptor
• UniProt Gene Name: PROKR1
• UniProt Synonym Gene Names: GPR73; PKR1; PK-R1
• UniProt Entry Name: PKR1_HUMAN

Uniprot Description

PROKR1: Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: neuropeptide Y receptor activity

Biological Process: neuropeptide signaling pathway; negative regulation of apoptosis