Typical Testing Data/Standard Curve (for reference only)
Sheep Prokineticin Receptor 1 ELISA Kit | PKR1 elisa kit
Sheep Prokineticin Receptor 1 ELISA Kit
Gene Names
PROKR1; ZAQ; PKR1; GPR73; GPR73a
Reactivity
Sheep
Synonyms
Prokineticin Receptor 1; Sheep Prokineticin Receptor 1 ELISA Kit; PKR1 elisa kit
Reactivity
Sheep
Samples
Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PKR1 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This PKR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PKR1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY PKR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKR1 antibody and an PKR1-HRP conjugate. The assay sample and buffer are incubated together with PKR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKR1 concentration since PKR1 from samples and PKR1-HRP conjugate compete for the anti-PKR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PKR1 from the sample, fewer sites are left to bind PKR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKR1 concentration in each sample is interpolated from this standard curve.
INTENDED USE This PKR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PKR1. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY PKR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKR1 antibody and an PKR1-HRP conjugate. The assay sample and buffer are incubated together with PKR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKR1 concentration since PKR1 from samples and PKR1-HRP conjugate compete for the anti-PKR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PKR1 from the sample, fewer sites are left to bind PKR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,770 Da
NCBI Official Full Name
prokineticin receptor 1
NCBI Official Synonym Full Names
prokineticin receptor 1
NCBI Official Symbol
PROKR1
NCBI Official Synonym Symbols
ZAQ; PKR1; GPR73; GPR73a
NCBI Protein Information
prokineticin receptor 1; PK-R1; G protein-coupled receptor 73; G-protein coupled receptor 73; G protein-coupled receptor ZAQ; G-protein coupled receptor ZAQ
UniProt Protein Name
Prokineticin receptor 1
Protein Family
UniProt Gene Name
PROKR1
UniProt Synonym Gene Names
GPR73; PKR1; PK-R1
UniProt Entry Name
PKR1_HUMAN
Uniprot Description
PROKR1: Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: plasma membrane; integral to membrane
Molecular Function: neuropeptide Y receptor activity
Biological Process: neuropeptide signaling pathway; negative regulation of apoptosis
Research Articles on PKR1
Similar Products
Product Notes
The Sheep PKR1 prokr1 (Catalog #AAA737289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737289 ELISA Kit recognizes Sheep PKR1. It is sometimes possible for the material contained within the vial of "Prokineticin Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
