Rat Prokineticin Receptor 1 ELISA Kit | PKR1 elisa kit

Rat Prokineticin Receptor 1 ELISA Kit

Cat. Num. AAA761114

• Gene Names: PROKR1; ZAQ; PKR1; GPR73; GPR73a
• Reactivity: Rat
• Synonyms: Prokineticin Receptor 1; Rat Prokineticin Receptor 1 ELISA Kit; PKR1/GPR73/GPR73a/PKR1/PROKR1/ZAQ/G protein-coupled receptor 73/G protein-coupled receptor ZAQ/GPR73/GPR73aG-protein coupled receptor 73/PK-R1/PKR1G-protein coupled receptor ZAQ/prokineticin receptor 1/ZAQ; PKR1 elisa kit

• Reactivity: Rat
• Sequence Length: 393

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PKR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat PKR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat PKR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat PKR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat PKR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20373179
• NCBI GeneID: 10887
• NCBI Accession #: NP_620414.1
• NCBI GenBank Nucleotide #: NM_138964.2
• Molecular Weight: 44,770 Da
• NCBI Official Full Name: prokineticin receptor 1
• NCBI Official Synonym Full Names: prokineticin receptor 1
• NCBI Official Symbol: PROKR1
• NCBI Official Synonym Symbols: ZAQ; PKR1; GPR73; GPR73a
• NCBI Protein Information: prokineticin receptor 1
• UniProt Protein Name: Prokineticin receptor 1
• Protein Family: Prokineticin receptor
• UniProt Gene Name: PROKR1
• UniProt Synonym Gene Names: GPR73; PKR1; PK-R1
• UniProt Entry Name: PKR1_HUMAN

Uniprot Description

PROKR1: Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide Y receptor activity

Biological Process: cell surface receptor linked signal transduction; circadian rhythm; G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis; neuropeptide signaling pathway