Typical Testing Data/Standard Curve (for reference only)
Pig collagen type I (COLI) ELISA Kit | COLI elisa kit
Pig collagen type I, COLI ELISA Kit
Reactivity
Pig
Synonyms
collagen type I (COLI); Pig collagen type I; COLI ELISA Kit; Pig collagen type I (COLI) ELISA kit; COLI elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of pig COLI. No significant cross-reactivity or interference between pig COLI and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
78 pg/ml -5000 pg/ml.
Sensitivity
The minimum detectable dose of pig COLI is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COLI elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COLI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COLI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COLI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COLI bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Product Notes
The Pig COLI (Catalog #AAA706558) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA706558 ELISA Kit recognizes Pig COLI. It is sometimes possible for the material contained within the vial of "collagen type I (COLI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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