Western Blot (WB)
(Anti-SMURF2 Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Rat SMURF2 Polyclonal Antibody | anti-SMURF2 antibody
SMURF2 Antibody (C-term)
IHC-P~~1:50~100
IF~~1:10~50
Western Blot (WB)
(Anti-SMURF2 Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-SMURF2 Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268))
Immunofluorescence (IF)
(Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268))
Immunofluorescence (IF)
(Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268))
Immunofluorescence (IF)
(Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268))
NCBI and Uniprot Product Information
Uniprot Description
SMURF2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ligase
Chromosomal Location of Human Ortholog: 17q22-q23
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus; cytosol; ubiquitin ligase complex; lipid raft
Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity; SMAD binding; transforming growth factor beta receptor binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; negative regulation of transcription, DNA-dependent
Research Articles on SMURF2
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Product Notes
The SMURF2 smurf2 (Catalog #AAA9205919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 702-731. The SMURF2 Antibody (C-term) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMURF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF). WB~~1:2000 IHC-P~~1:50~100 IF~~1:10~50. Researchers should empirically determine the suitability of the SMURF2 smurf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMURF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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