Mouse cathepsin L1 ELISA Kit | CTSL1 elisa kit

Mouse Cathepsin L1, CTSL1 ELISA Kit

Cat. Num. AAA900575

• Gene Names: Ctsl; fs; MEP; nkt; Ctsl1; 1190035F06Rik
• Reactivity: Mouse
• Synonyms: cathepsin L1; Mouse Cathepsin L1; CTSL1 ELISA Kit; Mouse Cathepsin L1 (CTSL1) ELISA kit; CATL; CTSL; FLJ31037; MEP; cathepsin L; major excreted protein; CTSL1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse CTSL1. No significant cross-reactivity or interference between mouse CTSL1 and analogues was observed.
• Sequence Length: 334

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse CTSL1 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSL1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6753558
• NCBI GeneID: 13039
• NCBI Accession #: NP_034114.1
• NCBI GenBank Nucleotide #: NM_009984.3
• UniProt Accession #: P06797; Q91UZ0
• Molecular Weight: 37,547 Da
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: Ctsl
• NCBI Official Synonym Symbols: fs; MEP; nkt; Ctsl1; 1190035F06Rik
• NCBI Protein Information: cathepsin L1; Cat L; nackt; furless; major excreted protein; p39 cysteine proteinase
• UniProt Protein Name: Cathepsin L1
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsl
• UniProt Synonym Gene Names: Ctsl1; MEP
• UniProt Entry Name: CATL1_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.22.15

Cellular Component: extracellular space; neuron projection; microvillus; lysosome; apical part of cell; cytoplasm; nucleolus; vacuole; perikaryon; cytoplasmic vesicle; nucleus; external side of plasma membrane; secretory granule

Molecular Function: peptidase activity; protein binding; hydrolase activity; histone binding; cysteine-type endopeptidase activity; protein complex binding; kininogen binding; cysteine-type carboxypeptidase activity; aminopeptidase activity; peptide binding; cysteine-type peptidase activity

Biological Process: protein autoprocessing; catagen; hair follicle morphogenesis; cell communication; protein processing; decidualization; proteolysis