Mouse tryptase beta 2 ELISA Kit | TPSB2 elisa kit

Mouse Tryptase beta-2, TPSB2 ELISA Kit

Cat. Num. AAA945041

• Reactivity: Mouse
• Synonyms: tryptase beta 2; Mouse Tryptase beta-2; TPSB2 ELISA Kit; Mouse Tryptase beta-2 (TPSB2) ELISA kit; TPS2; tryptaseB; tryptaseC; OTTHUMP00000208944; mast cell tryptase beta II; mast cell tryptase beta III; tryptase II; tryptase III; tryptase beta II; tryptase beta III; TPSB2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse TPSB2. No significant cross-reactivity or interference between mouse TPSB2 and analogues was observed.
• Sequence Length: 276

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 78 pg/ml -5000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse TPSB2 is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPSB2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPSB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126839
• UniProt Accession #: P21845; Q61962
• Molecular Weight: 30,927 Da
• NCBI Official Full Name: Tryptase beta-2
• UniProt Protein Name: Tryptase beta-2
• Protein Family: Tryptase
• UniProt Gene Name: Tpsb2
• UniProt Synonym Gene Names: Mcpt6; Tryptase-2; mMCP-6
• UniProt Entry Name: TRYB2_MOUSE

Uniprot Description

TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.59

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: peptidase activity; heparin binding; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: proteolysis; inflammatory response