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Western Blot (WB) (Nucleolin antibody (MBS839516) used at 1.25 ug/ml to detect target protein.)

Rabbit Nucleolin Polyclonal Antibody | anti-NCL antibody

Nucleolin antibody

Gene Names
NCL; C23
Applications
Western Blot, Immunohistochemistry
Purity
Total IgG Protein A purified
Synonyms
Nucleolin; Polyclonal Antibody; Nucleolin antibody; Polyclonal Nucleolin; Anti-Nucleolin; NCL; anti-NCL antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Specificity
Nucleolin antibody was raised against the C terminal of NCL
Purity/Purification
Total IgG Protein A purified
Form/Format
Purified antibody supplied as a liquid in 1X PBS buffer with 0.09% NaN3 , and 2% Sucrose.
Concentration
1 mg/ml (varies by lot)
Sequence Length
710
Applicable Applications for anti-NCL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1.25 ug/ml
IHC: 4-8 ug/ml
Biological Significance
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Cross-Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig , Horse, Rabbit
Immunogen
Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Preparation and Storage
Store at 4°C for short term storage. For long term Storage, store at -20°C. Avoid repeated freeze-thaws.

Western Blot (WB)

(Nucleolin antibody (MBS839516) used at 1.25 ug/ml to detect target protein.)

Western Blot (WB) (Nucleolin antibody (MBS839516) used at 1.25 ug/ml to detect target protein.)

Immunohistochemistry (IHC)

(Nucleolin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X.)

Immunohistochemistry (IHC) (Nucleolin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X.)
Product Categories/Family for anti-NCL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78 kDa (MW of target protein)
NCBI Official Full Name
nucleolin
NCBI Official Synonym Full Names
nucleolin
NCBI Official Symbol
NCL
NCBI Official Synonym Symbols
C23
NCBI Protein Information
nucleolin
UniProt Protein Name
Nucleolin
Protein Family
UniProt Gene Name
NCL
UniProt Entry Name
NUCL_HUMAN

NCBI Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Uniprot Description

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Nuclear receptor co-regulator; RNA-binding; Nucleolus; DNA-binding; Helicase

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; nucleus; ribonucleoprotein complex

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: angiogenesis

Research Articles on NCL

Similar Products

Product Notes

The NCL ncl (Catalog #AAA839516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nucleolin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1.25 ug/ml IHC: 4-8 ug/ml. Researchers should empirically determine the suitability of the NCL ncl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleolin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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