c-Jun, aa1-79, GST Fusion Protein Peptide | C-JUN peptide

c-Jun, aa1-79, GST Fusion Protein

Cat. Num. AAA658552

• Gene Names: C-JUN; Jun
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Immunoaffinity chromatography. 90%
• Synonyms: c-Jun; aa1-79; GST Fusion Protein; C-JUN peptide

• Purity/Purification: Affinity Purified; Purified by Immunoaffinity chromatography. 90%
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM EDTA, 1mM DTT, 50% glycerol.

• Applicable Applications for C-JUN peptide: Western Blot (WB)
• Activity: C-Jun fusion protein at a concentration of 2ug/20ul reaction was phosphorylated in an in vitro kinase assay with 200uM ATP. After 30 minutes incubation at 30 degree C, phosphorylation was detected by Western blot with phospho-specific c-Jun antibodies.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for C-JUN peptide

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activates c-Jun.

Product Categories/Family for C-JUN peptide

Molecular Biology; MB-Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1911701
• NCBI GeneID: 396913
• UniProt Accession #: P56432
• Molecular Weight: 37kD
• NCBI Official Full Name: c-Jun
• NCBI Official Symbol: C-JUN
• NCBI Official Synonym Symbols: Jun
• NCBI Protein Information: transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_PIG

Uniprot Description

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation

By similarity.

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7

By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex

By similarity. Interacts with RNF187 and HIPK3

By similarity.

Subcellular location: Nucleus

By similarity.

Post-translational modification: Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity

By similarity.Acetylated at Lys-271 by EP300

By similarity.

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP (basic-leucine zipper) domain.