Rabbit anti-Human c-Jun Polyclonal Antibody | anti-c-Jun antibody

c-Jun antibody

Cat. Num. AAA840826

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: c-Jun; Polyclonal Antibody; c-Jun antibody; JUN; AP1; p39; anti-c-Jun antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-human c-Jun polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 331

• Applicable Applications for anti-c-Jun antibody: Western Blot (WB)
• Application Notes: The antibody can be used in Western Blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant human c-Jun can be used as a positive control.
• Immunogen: E. coli-expressed recombinant human c-Jun fusion protein
• Positive Control: Recombinant human c-Jun
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-c-Jun antibody

Background: c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.

Product Categories/Family for anti-c-Jun antibody

Stem Cell Research Tools; Stem Cell Signaling Pathways; MAPK/ERK Signaling; AntibodiesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4758616
• NCBI GeneID: 3725
• NCBI Accession #: NP_002219.1
• NCBI GenBank Nucleotide #: NM_002228.3
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: transcription factor AP-1
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: nucleoplasm; nuclear chromosome; transcription factor complex; transcriptional repressor complex; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: circadian rhythm; positive regulation of monocyte differentiation; transcription from RNA polymerase II promoter; response to cAMP; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; microglial cell activation; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; SMAD protein nuclear translocation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; response to radiation; positive regulation of fibroblast proliferation; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; positive regulation of neuron apoptosis; negative regulation of protein amino acid autophosphorylation; negative regulation of neuron apoptosis; angiogenesis; toll-like receptor 4 signaling pathway; aging; response to drug; release of cytochrome c from mitochondria; monocyte differentiation; leading edge cell differentiation; axon regeneration; MyD88-independent toll-like receptor signaling pathway; learning; liver development; negative regulation of DNA binding; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; membrane depolarization; cellular response to potassium ion starvation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication