Rabbit c-Jun Polyclonal Antibody | anti-C-JUN antibody

c-Jun, phosphorylated (Ser63) (BSA, Azide and Glycerol Free)

Cat. Num. AAA629098

• Gene Names: C-JUN; Jun
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: c-Jun; Polyclonal Antibody; phosphorylated (Ser63) (BSA; Azide and Glycerol Free); Anti -c-Jun; anti-C-JUN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of human c-Jun only when phosphorylated at Ser63. Does not recognize the phosphorylated forms of JunD or JunB. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in PBS. No preservative added. No stabilizing proteins added.

• Applicable Applications for anti-C-JUN antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Flow Cytometry: 1:100; Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunohistochemistry (Paraffin): 1:50; Immunocytochemistry (ABC): 1:1000
• Immunogen: Synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from anisomycin or UV-treated NIH/3T3 cells, using MBS629098.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using MBS629098.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using MBS629098.)

Immunohistochemistry (IHC)

(Immunohistochemical analysisof paraffin-embedded human breast carcinoma, using MBS629098 preincubated with irrelevant control peptide (left) or Blocking Peptide C5815-10A(right).)

Immunocytochemistry (ICC)

(Immunocytochemical analysis of NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using MBS629098.)

Related Product Information for anti-C-JUN antibody

c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c- Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c- Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stressinduced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the N-terminal region of c- Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

Product Categories/Family for anti-C-JUN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1911701
• NCBI GeneID: 396913
• UniProt Accession #: P56432
• Molecular Weight: 35,629 Da
• NCBI Official Full Name: c-Jun
• NCBI Official Symbol: C-JUN
• NCBI Official Synonym Symbols: Jun
• NCBI Protein Information: transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Entry Name: JUN_PIG

Uniprot Description

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7

By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex

By similarity. Interacts with RNF187

Subcellular location: Nucleus

Post-translational modification: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP domain.