c-JUN recombinant protein

c-JUN recombinant protein

Cat. Num. AAA515154

• Gene Names: JUN; AP1; AP-1; c-Jun
• Applications: Western Blot
• Synonyms: c-JUN; c-JUN recombinant protein; AP1

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: 1-79
• Sequence Length: 3338

• Applicable Applications for c-JUN recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant human c-JUN (1-79) expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for c-JUN recombinant protein

Recombinant human c-JUN (1-79) was expressed in E.coli cells using a N-terminal GST tag.

Scientific Background: c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). c-Jun has specific DNA binding activity and following in vitro translation, c-Jun binds as a homodimer to the AP-1 DNA site. The transactivating function of c-Jun is acutely regulated by a wide variety of cellular signals via modulation of phosphorylation of two serines (63 and 73). Jun N-terminal domain kinases (JNKs), are responsible for mediating S63/73 phosphorylation on c-Jun in response to a variety of cellular stimuli including TNF-a, heat stress and u.v. light (2).

Product Categories/Family for c-JUN recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins

References

1. Halazonetis, T D. et al: c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinitie. Cell. 1988 Dec 2;55(5):917-24.
2. Dai, T. et al: Stress-activated protein kinases bind directly to the delta domain of c-Jun in resting cells: implications for repression of c-Jun function. Oncogene. 1995 Mar 2;10(5):849-55.

NCBI and Uniprot Product Information

• NCBI GI #: 44890066
• NCBI GeneID: 3725
• NCBI Accession #: NP_002219.1
• NCBI GenBank Nucleotide #: NM_002228
• UniProt Accession #: P05412; Q96G93
• Molecular Weight: ~35 kDa
• NCBI Official Full Name: Homo sapiens jun proto-oncogene (JUN), mRNA
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1; p39; jun oncogene; activator protein 1; proto-oncogene c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; v-jun sarcoma virus 17 oncogene homolog; v-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Ref.15 Ref.19

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA

By similarity. Interacts with HIVEP3 and MYBBP1A

By similarity. Interacts with SP1, SPIB and TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex. Interacts with RNF187. Binds to HIPK3. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.20 Ref.26

Subcellular location: Nucleus.

Post-translational modification: Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Ref.7 Ref.8 Ref.9 Ref.19 Ref.21 Ref.22 Ref.28 Ref.30Acetylated at Lys-271 by EP300.

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP (basic-leucine zipper) domain.