Bovine cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit | PDE5A elisa kit

Bovine cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit

Cat. Num. AAA288160

• Reactivity: Bovine
• Synonyms: cGMP-specific 3'; 5'-cyclic phosphodiesterase; Bovine cGMP-specific 3'; 5'-cyclic phosphodiesterase ELISA Kit; PDE5A elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovine cGMP-specific 3',5'-cyclic phosphodiesterase. No significant cross-reactivity or interference was observed.
• Sequence Length: 821

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 ng/ml -10.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine cGMP-specific 3',5'-cyclic phosphodiesterase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDE5A elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to cGMP-specific 3',5'-cyclic phosphodiesterase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for cGMP-specific 3',5'-cyclic phosphodiesterase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain cGMP-specific 3',5'-cyclic phosphodiesterase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of cGMP-specific 3',5'-cyclic phosphodiesterase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27806055
• NCBI GeneID: 281972
• NCBI Accession #: NP_776842.1
• NCBI GenBank Nucleotide #: NM_174417.2
• UniProt Accession #: Q28156
• Molecular Weight: 98,627 Da
• NCBI Official Full Name: cGMP-specific 3',5'-cyclic phosphodiesterase
• NCBI Official Symbol: PDE5A
• NCBI Protein Information: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Protein Name: cGMP-specific 3',5'-cyclic phosphodiesterase
• Protein Family: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE5A
• UniProt Synonym Gene Names: PDE5; CGB-PDE
• UniProt Entry Name: PDE5A_BOVIN

Uniprot Description

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity

Biological Process: cGMP catabolic process