Dog Heat shock 70 kDa protein 1 ELISA Kit | HSPA1 elisa kit

Dog Heat shock 70 kDa protein 1 ELISA Kit

Cat. Num. AAA288454

• Gene Names: HSP70; HSPA1
• Reactivity: Dog
• Synonyms: Heat shock 70 kDa protein 1; Dog Heat shock 70 kDa protein 1 ELISA Kit; HSPA1 elisa kit

• Reactivity: Dog
• Specificity: This assay recognizes recombinant and natural dog Heat shock 70 kDa protein 1. No significant cross-reactivity or interference was observed.
• Sequence Length: 641

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 ng/ml -10.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of dog Heat shock 70 kDa protein 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSPA1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat shock 70 kDa protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Heat shock 70 kDa protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock 70 kDa protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Heat shock 70 kDa protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 924859494
• NCBI GeneID: 403612
• NCBI Accession #: NP_001003067.2
• NCBI GenBank Nucleotide #: NM_001003067.2
• UniProt Accession #: Q7YQC6; Q8WNS9
• Molecular Weight: 70,070 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1
• NCBI Official Symbol: HSP70
• NCBI Official Synonym Symbols: HSPA1
• NCBI Protein Information: heat shock 70 kDa protein 1
• UniProt Protein Name: Heat shock 70 kDa protein 1
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA1
• UniProt Synonym Gene Names: Hsp70
• UniProt Entry Name: HSP71_CANLF

Uniprot Description

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.