Bovine Galectin-9 ELISA Kit | LGALS9 elisa kit

Bovine Galectin-9 ELISA Kit

Cat. Num. AAA288304

• Reactivity: Bovine
• Synonyms: Galectin-9; Bovine Galectin-9 ELISA Kit; LGALS9 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovine Gal-9. No significant cross-reactivity or interference was observed.
• Sequence Length: 323

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 ng/ml -10.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine Gal-9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS9 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gal-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Gal-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gal-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gal-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 87044899
• NCBI GeneID: 510813
• NCBI Accession #: NP_001034266.1
• NCBI GenBank Nucleotide #: NM_001039177.2
• UniProt Accession #: Q3MHZ8
• Molecular Weight: 39,334 Da
• NCBI Official Full Name: galectin-9 isoform 1
• NCBI Official Symbol: LGALS9
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• Protein Family: Galectin
• UniProt Gene Name: LGALS9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_BOVIN

Uniprot Description

Binds galactosides. Has high affinity for the Forssman pentasaccharide. Ligand for HAVCR2/TIM3. Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death. Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth. Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration. Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function. Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation. Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection. Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells. Inhibits degranulation and induces apoptosis of mast cells. Induces maturation and migration of dendritic cells. Inhibits natural killer (NK) cell function. Can transform NK cell phenotype from peripheral to decidual during pregnancy. Astrocyte derived galectin-9 enhances microglial TNF production. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion. Highly selective to the anion urate.