Bovine NLR family CARD domain-containing protein 4 ELISA Kit | NLRC4 elisa kit

Bovine NLR family CARD domain-containing protein 4 ELISA Kit

Cat. Num. AAA288261

• Reactivity: Bovine
• Synonyms: NLR family CARD domain-containing protein 4; Bovine NLR family CARD domain-containing protein 4 ELISA Kit; NLRC4 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovine NLR family CARD domain-containing protein 4. No significant cross-reactivity or interference was observed.
• Sequence Length: 1024

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 0.15 ng/ml -10.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine NLR family CARD domain-containing protein 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NLRC4 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR family CARD domain-containing protein 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NLR family CARD domain-containing protein 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NLR family CARD domain-containing protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NLR family CARD domain-containing protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 328927036
• NCBI GeneID: 512480
• NCBI Accession #: NP_001179252.2
• NCBI GenBank Nucleotide #: NM_001192323.2
• UniProt Accession #: F1MHT9
• Molecular Weight: 115,578 Da
• NCBI Official Full Name: NLR family CARD domain-containing protein 4
• NCBI Official Symbol: NLRC4
• NCBI Protein Information: NLR family CARD domain-containing protein 4
• UniProt Protein Name: NLR family CARD domain-containing protein 4
• Protein Family: NLR family CARD domain-containing protein
• UniProt Gene Name: NLRC4
• UniProt Synonym Gene Names: IPAF; Ipaf
• UniProt Entry Name: NLRC4_BOVIN

Uniprot Description

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.