Western Blot (WB)
(Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.)
Mouse Neuroligin 3 Monoclonal Antibody | anti-Nlgn3 antibody
Neuroligin 3 Antibody: FITC
Western Blot (WB)
(Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.)
Western Blot (WB)
(Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.)
Scientific Background: Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.
NCBI and Uniprot Product Information
NCBI Description
neuronal cell surface protein that binds neurexins [RGD, Feb 2006]
Uniprot Description
NLGN3: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Defects in NLGN3 may be the cause of susceptibility to autism X-linked type 1 (AUTSX1). AUTSX1 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Defects in NLGN3 may be the cause of susceptibility to X- linked Asperger syndrome 1 (ASPGX1). ASPGX1 is considered to be a form of childhood autism. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cell surface; endocytic vesicle; integral to plasma membrane; excitatory synapse; synapse; cell junction
Molecular Function: neurexin binding; receptor activity; cell adhesion molecule binding
Biological Process: regulation of synaptic transmission; receptor-mediated endocytosis; rhythmic synaptic transmission; regulation of synaptic transmission, glutamatergic; axon extension; metabolic process; neurological control of breathing; learning; social behavior; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; regulation of inhibitory postsynaptic membrane potential; synaptogenesis; adult behavior; synapse organization and biogenesis; oligodendrocyte differentiation; visual learning; neuron adhesion; cell adhesion; regulation of excitatory postsynaptic membrane potential
Research Articles on Nlgn3
Similar Products
Product Notes
The Nlgn3 nlgn3 (Catalog #AAA801373) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neuroligin 3 Antibody: FITC reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuroligin 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). 1:1000 WB. Researchers should empirically determine the suitability of the Nlgn3 nlgn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuroligin 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
