PCOLCE recombinant protein

PCOLCE, 26-449aa, Human, His-tag, Baculovirus

Cat. Num. AAA206814

• Gene Names: PCOLCE; PCPE; PCPE1; PCPE-1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PCOLCE; 26-449aa; Human; His-tag; Baculovirus; Procollagen C-endopeptidase enhancer 1; PCOLCE PCPE; PCPE-1; PCPE1; PCOLCE recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol, 0.1M NaCl.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPQTPNYTR PVFLCGGDVK GESGYVASEG FPNLYPPNKE CIWTITVPEG QTVSLSFRVF DLELHPACRY DALEVFAGSG TSGQRLGRFC GTFRPAPLVA PGNQVTLRMT TDEGTGGRGF LLWYSGRATS GTEHQFCGGR LEKAQGTLTT PNWPESDYPP GISCSWHIIA PPDQVIALTF EKFDLEPDTY CRYDSVSVFN GAVSDDSRRL GKFCGDAVPG SISSEGNELL VQFVSDLSVT ADGFSASYKT LPRGTAKEGQ GPGPKRGTEP KVKLPPKSQP PEKTEESPSA PDAPTCPKQC RRTGTLQSNF CASSLVVTAT VKSMVREPGE GLAVTVSLIG AYKTGGLDLP SPPTGASLKF YVPCKQCPPM KKGVSYLLMG QVEENRGPVL PPESFVVLHR PNQDQILTNL SKRKCPSQPV RAAASQDHHH HHH
• Sequence Length: 449

• Applicable Applications for PCOLCE recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PCOLCE recombinant protein

PCOLCE, also known as procollagen C-endopeptidase enhancer 1, is a secreted extracellular matrix glycoprotein. This protein is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin. Also, fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. Also, this protein binds and drives the enzymatic cleavage of type 1 procollagen and heightens C-proteinase activity. Recombinant human PCOLCE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for PCOLCE recombinant protein

Signal Transduction; Cytoskeleton

References

Takahara K., et al, (1994) J. Biol. Chem. 269:26280-26285. Scott IC., et al, (1999) Genomics 55:229-234.

NCBI and Uniprot Product Information

• NCBI GI #: 157653329
• NCBI GeneID: 5118
• NCBI Accession #: NP_002584
• NCBI GenBank Nucleotide #: NM_002593.3
• UniProt Accession #: Q15113; O14550; B2R9E1
• Molecular Weight: 46.6kDa (433aa) 40-57kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: procollagen C-endopeptidase enhancer 1
• NCBI Official Synonym Full Names: procollagen C-endopeptidase enhancer
• NCBI Official Symbol: PCOLCE
• NCBI Official Synonym Symbols: PCPE; PCPE1; PCPE-1
• NCBI Protein Information: procollagen C-endopeptidase enhancer 1
• UniProt Protein Name: Procollagen C-endopeptidase enhancer 1
• Protein Family: Procollagen C-endopeptidase enhancer
• UniProt Gene Name: PCOLCE
• UniProt Synonym Gene Names: PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1

NCBI Description

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.