Rabbit ATF6 Antibody | anti-ATF6 antibody

ATF6 Antibody

Cat. Num. AAA857165

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: ATF6; ATF6 Antibody; HIF1a; ARNT interacting protein; HIF 1 alpha; HIF 1alpha; PASD8; MOP1; anti-ATF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 850

• Applicable Applications for anti-ATF6 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:100; IF: 1:20-1:50
• Immunogen: Recombinant protein of Human Hif1a
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-ATF6 antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1alpha and HIF-1beta, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is prolinehydroxylated leading to a conformational change that promotes binding to the von HippelLindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1beta is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1beta plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1beta expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1beta plays an important role in pancreatic beta-cell function (10).

Product Categories/Family for anti-ATF6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. 2. Wang, G.L. et al. (1995) ProcNatlAcadSci U S A 92, 5510-4. 3. Jaakkola, P. et al. (2001) Science 292, 468-72. 4. Maxwell, P.H. et al. (1999) Nature 399, 271-5. 5. Fukuda, R. et al. (2002) J BiolChem 277, 38205-11. 6. Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. 7. Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. 8. Walisser, J.A. et al. (2004) ProcNatlAcadSci U S A 101, 16677-82. 9. Salomon-Nguyen, F. et al. (2000) ProcNatlAcadSci U S A 97, 6757-62. 10. Gunton, J.E. et al. (2005) Cell 122, 337-49.

NCBI and Uniprot Product Information

• NCBI GI #: 340545531
• NCBI GeneID: 3091
• NCBI Accession #: NP_001230013.1
• NCBI GenBank Nucleotide #: NM_001243084.1
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1; C0LZJ3
• Molecular Weight: 93kDa
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 3
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 subunit alpha
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: bHLHe78

NCBI Description

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.