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Bioactivity
(Sirtuin 4, also known as SIRT4, is a member of the sirtuin family. SIRT4 is a mitochondrial ADP-ribosyltransferase that inhibits mitochondrial glutamate dehydrogenase 1 activity, thereby downregulating insulin secretion in response to amino acids. It has been shown that SIRT4 regulates fatty acid oxidation and mitochondrial gene expression in liver and muscle cells. Besides, Heat Shock 60kD Protein 1, Chaperonin (HSPD1) has been identified as an interactor of SIRT4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SIRT4 and recombinant human HSPD1. Briefly, SIRT4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to HSPD1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SIRT4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT4 and HSPD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SIRT4 with HSPD1)
Sirtuin 4 Active Protein | SIRT4 active protein
Active Sirtuin 4 (SIRT4)
Gene Names
SIRT4; SIR2L4
Applications
Activity Assay, Cell Culture
Purity
>97%
Synonyms
Sirtuin 4; N/A; Active Sirtuin 4 (SIRT4); SIR2L4; Silent Mating Type Information Regulation 2 Homolog 4; SIR2-like protein 4; NAD-dependent protein deacetylase sirtuin-4; SIRT4 active protein
Purity/Purification
>97%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Ser28~Cys314
Sequence Length
335
Applicable Applications for SIRT4 active protein
Activity Assay, Cell Culture
Species
Human
Tag
N-terminal His Tag
Isoelectric Point
8.8
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Bioactivity
(Sirtuin 4, also known as SIRT4, is a member of the sirtuin family. SIRT4 is a mitochondrial ADP-ribosyltransferase that inhibits mitochondrial glutamate dehydrogenase 1 activity, thereby downregulating insulin secretion in response to amino acids. It has been shown that SIRT4 regulates fatty acid oxidation and mitochondrial gene expression in liver and muscle cells. Besides, Heat Shock 60kD Protein 1, Chaperonin (HSPD1) has been identified as an interactor of SIRT4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SIRT4 and recombinant human HSPD1. Briefly, SIRT4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to HSPD1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SIRT4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT4 and HSPD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SIRT4 with HSPD1)
Bioactivity
(Sirtuin 4, also known as SIRT4, is a member of the sirtuin family. SIRT4 is a mitochondrial ADP-ribosyltransferase that inhibits mitochondrial glutamate dehydrogenase 1 activity, thereby downregulating insulin secretion in response to amino acids. It has been shown that SIRT4 regulates fatty acid oxidation and mitochondrial gene expression in liver and muscle cells. Besides, Heat Shock 60kD Protein 1, Chaperonin (HSPD1) has been identified as an interactor of SIRT4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SIRT4 and recombinant human HSPD1. Briefly, SIRT4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to HSPD1-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-SIRT4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT4 and HSPD1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of SIRT4 with HSPD1)
Gene Sequencing
(Figure. Gene Sequencing (Extract))
Gene Sequencing
(Figure. Gene Sequencing (Extract))
WB (Western Blot)
(Figure. Western Blot)
WB (Western Blot)
(Figure. Western Blot)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for SIRT4 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 35.9kDa
Accurate Molecular Mass: 36kDa
Accurate Molecular Mass: 36kDa
NCBI Official Full Name
sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), isoform CRA_a
NCBI Official Synonym Full Names
sirtuin 4
NCBI Official Symbol
SIRT4
NCBI Official Synonym Symbols
SIR2L4
NCBI Protein Information
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-4
UniProt Gene Name
SIRT4
UniProt Entry Name
SIR4_HUMAN
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Product Notes
The SIRT4 sirt4 (Catalog #AAA150139) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser28~Cys314. AAA Biotech's Sirtuin 4 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the SIRT4 sirt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sirtuin 4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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