METTL11A recombinant protein

Recombinant METTL11A (NTMT1) protein

Cat. Num. AAA389088

• Gene Names: NTMT1; NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
• Synonyms: METTL11A; Recombinant METTL11A (NTMT1) protein; Methyltransferase-Like Protein 11A; N-Terminal RCC1 Methyltransferase; N-Terminal Xaa-Pro-Lys N-Methyltransferase 1; METTL11A recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant METTL11A/NTMT1 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. N-terminal Methylation Assay Conditions: 3uM CENP-A peptide (GPRRRSRKPEAPRRR) was incubated with 50nm METTL11A protein in reaction buffer including 50mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 50uM SAM for 2 hours at room temperature. Activity was detected by MALDI-TOF. *represents an uncertainty peak. Capacity ability: 60 turnovers/enzyme molecule
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant METTL11A / NTMT1 protein gel 10% SDS-PAGE Coomassie staining MW: 27 kDa Purity: >80% )

Testing Data

(MALDI-TOF assay for METTL11A protein activity CENP-A peptide was incubated with METTL11A protein in reaction buffer for 2 hours at room temperature. Activity was detected by MALDI-TOF. * represents an uncertainty peak. Capacity ability: 60 turnovers / enzyme molecule)

Related Product Information for METTL11A recombinant protein

Short Description: Recombinant METTL11A/NTMT1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_054783.2) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 27 kDa. It is suitable for use in in binding assays, inhibitor screening, and selectivity profiling.

Background: METTL11A (Methyltransferase-Like Protein 11A)/NTMT1 (N-Terminal Xaa-Pro-Lys N-Methyltransferase 1), also known as NRMT1 or NTM1A, is a distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. It specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. METTL11A catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met- 1). It is also responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. And it is required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

Product Categories/Family for METTL11A recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 56676399
• NCBI GeneID: 28989
• NCBI Accession #: NP_054783.2
• NCBI GenBank Nucleotide #: NM_014064.3
• UniProt Accession #: Q9BV86; Q5SZB9; Q9UI28; A8K4J2; A8K8G7
• Molecular Weight: 15,896 Da
• NCBI Official Full Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1 isoform a
• NCBI Official Synonym Full Names: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• NCBI Official Symbol: NTMT1
• NCBI Official Synonym Symbols: NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
• NCBI Protein Information: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• UniProt Protein Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• UniProt Gene Name: NTMT1
• UniProt Synonym Gene Names: C9orf32; METTL11A; NRMT; NRMT1; NTM1A
• UniProt Entry Name: NTM1A_HUMAN

NCBI Description

The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).[supplied by OMIM, Nov 2010]

Uniprot Description

METTL11A: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]- Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di- methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. Belongs to the methyltransferase superfamily. NTM1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.244; Methyltransferase; Methyltransferase, protein N-term

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: histone methyltransferase activity; protein methyltransferase activity

Biological Process: chromosome segregation; histone methylation; N-terminal peptidyl-alanine methylation; N-terminal peptidyl-glycine methylation; N-terminal peptidyl-proline di-methylation; spindle organization and biogenesis