METTL11A recombinant protein

Recombinant Human METTL11A Protein (GST tag)

Cat. Num. AAA2545856

• Gene Names: NTMT1; NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: METTL11A; Recombinant Human METTL11A Protein (GST tag); RP11-483H20.8; AD-003; C9orf32; METTL11A recombinant protein

• Host: E Coli
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM tris 150mM NaCl, 0.5mM GSH 10% glycerol, pH 7.5
• Sequence Length: 223

• Application Notes: The recombinant human METTL11A/GST chimera consists of 454 amino acids and has a predicted molecular mass of 52.2 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for METTL11A recombinant protein

Background: Methyltransferase-like protein 11A, also known as METTL11A, is a member of the methyltransferase superfamily and METTL11 family. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Methylation often occurs on nucleic bases in DNA or amino acids in protein structures. Methytransferase uses a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. Methylation can serve to protect DNA from enzymatic cleavage, since restriction enzymes are unable to bind and recognize externally modified sequences. This is especially useful in bacterial restriction modification systems which use restriction enzymes to cleave foreign DNA while keeping their own DNA protected by methylation. Methylation of amino acids in the formation of proteins leads to more diversity of possible amino acids and therefore more diversity of function. The methylation reaction occurs on nitrogen atoms either on the N terminus or side-chain position of the protein and are usually irreversible.

Description: A DNA sequence encoding the human METTL11A (NP_054783.2) (Thr 2-Arg 223) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 56676399
• NCBI GeneID: 28989
• NCBI Accession #: NP_054783.2
• NCBI GenBank Nucleotide #: NM_014064.3
• UniProt Accession #: Q9BV86; Q5SZB9; Q9UI28; A8K4J2; A8K8G7
• Molecular Weight: 15,896 Da
• NCBI Official Full Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1 isoform a
• NCBI Official Synonym Full Names: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• NCBI Official Symbol: NTMT1
• NCBI Official Synonym Symbols: NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
• NCBI Protein Information: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• UniProt Protein Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1
• UniProt Gene Name: NTMT1
• UniProt Synonym Gene Names: C9orf32; METTL11A; NRMT; NRMT1; NTM1A

NCBI Description

The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).[supplied by OMIM, Nov 2010]

Uniprot Description

METTL11A: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]- Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di- methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. Belongs to the methyltransferase superfamily. NTM1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.244; Methyltransferase; Methyltransferase, protein N-term

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: histone methyltransferase activity; protein methyltransferase activity

Biological Process: chromosome segregation; histone methylation; N-terminal peptidyl-alanine methylation; N-terminal peptidyl-glycine methylation; N-terminal peptidyl-proline di-methylation; spindle organization and biogenesis