Rabbit PDIA4 / ERP72 Polyclonal Antibody | anti-PDIA4 antibody

Rabbit Polyclonal to Mouse PDIA4 / ERP72

Cat. Num. AAA243790

• Gene Names: PDIA4; ERP70; ERP72; ERp-72
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A Purified
• Synonyms: PDIA4 / ERP72; Polyclonal Antibody; Rabbit Polyclonal to Mouse PDIA4 / ERP72; Anti-PDIA4 / ERP72 Antibody (aa623-638) IHC-plus; PDIA4; ER protein 70; ERp-72; ERP70; ERP72; Protein disulfide-isomerase A4; ER protein 72; Mouse PDIA4; anti-PDIA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Detects an ~72 kD protein, corresponding to the apparent molecular mass of ERp72.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.09% azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 645

• Applicable Applications for anti-PDIA4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Mouse
• Immunogen Description: A 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Immunogen: PDIA4 / ERP72 antibody was raised against a 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster (94%); Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%); Pig, Platypus (81%).
• Antigen Modification: aa623-638
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-ERp72 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ERp72 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ERp72 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ERp72 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of ERp72: Lane 1: MW Marker, Lane 2: Rat Liver Microsome Extract, Lane 3: Mouse Liver Microsome Extract, Lane 4: Human Liver Microsome Extract.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758304
• NCBI GeneID: 9601
• NCBI Accession #: NP_004902.1
• NCBI GenBank Nucleotide #: NM_004911.4
• UniProt Accession #: P13667; Q549T6; A8K4K6
• Molecular Weight: 72,932 Da
• NCBI Official Full Name: protein disulfide-isomerase A4
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 4
• NCBI Official Symbol: PDIA4
• NCBI Official Synonym Symbols: ERP70; ERP72; ERp-72
• NCBI Protein Information: protein disulfide-isomerase A4; ER protein 70; ER protein 72; protein disulfide isomerase-associated 4; endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)
• UniProt Protein Name: Protein disulfide-isomerase A4
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA4
• UniProt Synonym Gene Names: ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
• UniProt Entry Name: PDIA4_HUMAN

Uniprot Description

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: Isomerase; EC 5.3.4.1; Endoplasmic reticulum; Chaperone

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; cell redox homeostasis; protein secretion