Neuropilin 2 / NRP2 Recombinant Protein | NRP2 recombinant protein

Recombinant Human Neuropilin 2 / NRP2 Protein (Fc tag)

Cat. Num. AAA2546135

• Gene Names: NRP2; NP2; NPN2; PRO2714; VEGF165R2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Neuropilin 2 / NRP2; Recombinant Human Neuropilin 2 / NRP2 Protein (Fc tag); NRP2; VEGF165R2; NRP2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 926

• Application Notes: The recombinant human NRP2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1072 amino acids and has a predicted molecular mass of 120.7 kDa. The apparent molecular mass of the protein is approximately 119-129 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NRP2 recombinant protein

Background: Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classIII semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as formation of small lymphatic vessels and capillaries. Recent study has identified NRP-2 as a polysialylated protein expressed in human dendritic cells and modulates DC-T cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis and metastasis.

Description: A DNA sequence encoding the human NRP2 (NP_003863.2) (Met1-Tyr855) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 41872533
• NCBI GeneID: 8828
• NCBI Accession #: NP_003863.2
• NCBI GenBank Nucleotide #: NM_003872.2
• UniProt Accession #: O60462; O14820; O14821; Q53TQ4; Q53TS3; Q7LBX7; Q9H2D4; Q9H2D5; Q9H2E2; Q9H2E3; Q9H2E4; E9PF66
• Molecular Weight: 62,458 Da
• NCBI Official Full Name: neuropilin-2 isoform 2
• NCBI Official Synonym Full Names: neuropilin 2
• NCBI Official Symbol: NRP2
• NCBI Official Synonym Symbols: NP2; NPN2; PRO2714; VEGF165R2
• NCBI Protein Information: neuropilin-2
• UniProt Protein Name: Neuropilin-2
• Protein Family: Neuropilin
• UniProt Gene Name: NRP2
• UniProt Synonym Gene Names: VEGF165R2

NCBI Description

This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NRP2: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. Heterodimer with NRP1. Binds PLXNB1. Belongs to the neuropilin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: membrane; plasma membrane

Molecular Function: growth factor binding; receptor activity

Biological Process: axon extension involved in axon guidance; nerve development; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway