Neuropilin-2 Active Protein | NRP2 active protein

Neuropilin-2 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120429

• Gene Names: NRP2; NP2; NPN2; PRO2714; VEGF165R2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Neuropilin-2; Neuropilin-2 Protein; Human; Recombinant (hFc Tag); Human Neuropilin 2/NRP2 Protein (Fc Tag); NP2 Protein; NPN2 Protein; PRO2714 Protein; VEGF165R2 Protein; neuropilin 2; NRP2 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr855

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 23
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NRP2 (NP_003863.2)(Met1-Tyr855) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1.Measured by its binding ability in a functional ELISA.2.Immobilized human VEGFC-His at 10ug/mL (100uL/well) can bind human NRP2-Fc. The EC50 of human NRP2-Fc is 0.1-0.5ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human VEGFC-His at 10 ug/mL (100 uL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5 ug/mL.)

Related Product Information for NRP2 active protein

Background: Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classI¢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as the formation of small lymphatic vessels and capillaries. A recent study has identified NRP-2 as a polysialylation protein expressed in human dendritic cells and modulates DC-T Cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis, and metastasis.

References

Chen H. et al., 1997, Neuron. 19: 547-59. Bielenberg DR. et al., 2006, Exp Cell Res. 312: 584-93. Yuan L. et al., 2002, Development. 129: 4797-806. Benoit F. et al., 2006, Blood. 108: 1243-50. Sabrina C. et al., 2007, J Biol Chem. 282: 30346-56. Gray MJ. et al., 2008, J Natl Cancer Inst. 100: 109-20.

NCBI and Uniprot Product Information

• NCBI GI #: 41872533
• NCBI GeneID: 8828
• NCBI Accession #: NP_003863.2
• NCBI GenBank Nucleotide #: NM_003872.2
• UniProt Accession #: O60462; O14820; O14821; Q53TQ4; Q53TS3; Q7LBX7; Q9H2D4; Q9H2D5; Q9H2E2; Q9H2E3; Q9H2E4; E9PF66
• Molecular Weight: 931
• NCBI Official Full Name: neuropilin-2 isoform 2
• NCBI Official Synonym Full Names: neuropilin 2
• NCBI Official Symbol: NRP2
• NCBI Official Synonym Symbols: NP2; NPN2; PRO2714; VEGF165R2
• NCBI Protein Information: neuropilin-2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2
• UniProt Protein Name: Neuropilin-2
• Protein Family: Neuropilin
• UniProt Gene Name: NRP2
• UniProt Synonym Gene Names: VEGF165R2
• UniProt Entry Name: NRP2_HUMAN

Uniprot Description

NRP2: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. Heterodimer with NRP1. Binds PLXNB1. Belongs to the neuropilin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; semaphorin receptor activity; cytokine binding; growth factor binding; metal ion binding; receptor activity

Biological Process: axon extension involved in axon guidance; axon guidance; positive regulation of endothelial cell proliferation; nerve development; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion