Rat nephrosis 1, congenital, Finnish type (nephrin) ELISA Kit | NPHS1 elisa kit

Rat Nephrin ELISA Kit

Cat. Num. AAA702948

• Reactivity: Rat
• Synonyms: nephrosis 1; congenital; Finnish type (nephrin); Rat Nephrin ELISA Kit; CNF; NPHN; nephrin; renal glomerulus-specific cell adhesion receptor; NPHS1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat Nephrin. No significant cross-reactivity or interference between rat Nephrin and analogues was observed.
• Sequence Length: 1252

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.25 ng/ml-80 ng/ml.
• Sensitivity: The minimum detectable dose of rat Nephrin is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPHS1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Nephrin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Nephrin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Nephrin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Nephrin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 12018318
• NCBI GeneID: 64563
• NCBI Accession #: NP_072150.1
• NCBI GenBank Nucleotide #: NM_022628.1
• UniProt Accession #: Q9R044; Q9JIX2; Q9QXX7
• Molecular Weight: 136,281 Da
• NCBI Official Full Name: nephrin
• NCBI Official Synonym Full Names: nephrosis 1, congenital, Finnish type (nephrin)
• NCBI Official Symbol: Nphs1
• NCBI Protein Information: nephrin; nephrosis 1 homolog, nephrin; renal glomerulus-specific cell adhesion receptor
• UniProt Protein Name: Nephrin
• Protein Family: Nephrin
• UniProt Gene Name: Nphs1
• UniProt Synonym Gene Names: Nphn
• UniProt Entry Name: NPHN_RAT

NCBI Description

renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]

Uniprot Description

Function: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.2 PublicationsManual assertion based on experiment in:Ref.3