ELISA (EIA)
(Sandwich ELISA analysis of human CD30 using Mouse anti Human CD30 as a capture reagent and biotinylated Mouse anti Human CD30 (MBS226114) as a detection reagent with purified recombinant CD30 as the antigen. Detection was performed using HRP co)
Mouse anti-Human CD30 Monoclonal Antibody | anti-CD30 antibody
Mouse anti Human CD30:Biotin
Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD30; Monoclonal Antibody; Mouse anti Human CD30:Biotin; CD30 antibody | D02-6E11; anti-CD30 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
D02-6E11
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD30 antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli derived recombinant human full length CD30 (Phe19-Lys595)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human CD30 using Mouse anti Human CD30 as a capture reagent and biotinylated Mouse anti Human CD30 (MBS226114) as a detection reagent with purified recombinant CD30 as the antigen. Detection was performed using HRP co)
ELISA (EIA)
(Sandwich ELISA analysis of human CD30 using Mouse anti Human CD30 as a capture reagent and biotinylated Mouse anti Human CD30 (MBS226114) as a detection reagent with purified recombinant CD30 as the antigen. Detection was performed using HRP co)
Related Product Information for anti-CD30 antibody
Mouse anti Human CD30 antibody, clone D02-6E11, recognizes CD30 also known as Tumor Necrosis Factor Receptor Super Family member 8, CD30L receptor and Ki-1 antigen. CD30 is a 120kDa transmembrane glycoprotein that belongs to the tumor necrosis factor receptor superfamily. CD30 is expressed on small subsets of activated T and B lymphocytes and a range of lymphoid neoplasms (Weyden et al. 2017).
Upon binding of its ligand CD30L, CD30 undergoes trimerization and recruits TNFR-associated factor (TRAF) and TRAF binding proteins generating a signaling complex which can then mediate MAPK and NFkappaB signaling (Weyden et al. 2017). In this way, CD30 signaling is able to regulate peripheral T cell responses, controls T cell survival, down regulates cytolitic capacity and regulates thymocyte survival (Muta & Podack 2013).
The biotinylated Mouse anti Human CD30 antibody, clone D02-6E11 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD30 antibody, clone G05-2D2 as the capture antibody.
Upon binding of its ligand CD30L, CD30 undergoes trimerization and recruits TNFR-associated factor (TRAF) and TRAF binding proteins generating a signaling complex which can then mediate MAPK and NFkappaB signaling (Weyden et al. 2017). In this way, CD30 signaling is able to regulate peripheral T cell responses, controls T cell survival, down regulates cytolitic capacity and regulates thymocyte survival (Muta & Podack 2013).
The biotinylated Mouse anti Human CD30 antibody, clone D02-6E11 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CD30 antibody, clone G05-2D2 as the capture antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,935 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 8 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 8
NCBI Official Symbol
TNFRSF8
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
Protein Family
UniProt Gene Name
TNFRSF8
UniProt Synonym Gene Names
CD30; D1S166E
UniProt Entry Name
TNR8_HUMAN
Uniprot Description
TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: integral to plasma membrane
Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity
Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction
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Product Notes
The CD30 tnfrsf8 (Catalog #AAA226114) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CD30:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the CD30 tnfrsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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