Typical Testing Data/Standard Curve (for reference only)
Rat Nephrin (NPHN) RTU ELISA Kit | NPHN rtu elisa kit
Rat Nephrin (NPHN) ELISA Kit
Reactivity
Rat
Synonyms
Nephrin (NPHN); Rat Nephrin (NPHN) ELISA Kit; NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor; NPHN rtu elisa kit
Reactivity
Rat
Sequence Length
1252
Assay Type
Sandwich
Samples
Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.112ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NPHN rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPHN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NPHN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,646 Da
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
NPHS1 nephrin
NCBI Official Symbol
Nphs1
NCBI Protein Information
nephrin
UniProt Protein Name
Nephrin
UniProt Gene Name
Nphs1
UniProt Synonym Gene Names
Nphn
NCBI Description
renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]
Uniprot Description
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Research Articles on NPHN
Similar Products
Product Notes
The Rat NPHN nphs1 (Catalog #AAA4501429) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4501429 RTU ELISA Kit recognizes Rat NPHN. It is sometimes possible for the material contained within the vial of "Nephrin (NPHN), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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