Human dipeptidase 1 (renal) ELISA Kit | DPEP1 elisa kit

Human Dipeptidase 1, DPEP1 ELISA Kit

Cat. Num. AAA911972

• Gene Names: DPEP1; MDP; RDP; MBD1
• Reactivity: Human
• Synonyms: dipeptidase 1 (renal); Human Dipeptidase 1; DPEP1 ELISA Kit; Human Dipeptidase 1 (DPEP1) ELISA kit; MBD1; MDP; RDP; OTTHUMP00000175356; dipeptidase 1; DPEP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DPEP1. No significant cross-reactivity or interference between human DPEP1 and analogues was observed.
• Sequence Length: 411

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 23.5 pg/ml -1500 pg/ml.
• Sensitivity: The minimum detectable dose of human DPEP1 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPEP1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPEP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPEP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPEP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 189458885
• NCBI GeneID: 1800
• NCBI Accession #: NP_001121613.1
• NCBI GenBank Nucleotide #: NM_001128141.2
• UniProt Accession #: P16444; Q96AK2; D3DX80
• Molecular Weight: 45,674 Da
• NCBI Official Full Name: dipeptidase 1
• NCBI Official Synonym Full Names: dipeptidase 1 (renal)
• NCBI Official Symbol: DPEP1
• NCBI Official Synonym Symbols: MDP; RDP; MBD1
• NCBI Protein Information: dipeptidase 1; hRDP; renal dipeptidase; dehydropeptidase-I; microsomal dipeptidase
• UniProt Protein Name: Dipeptidase 1
• Protein Family: Dipeptidase
• UniProt Gene Name: DPEP1
• UniProt Synonym Gene Names: MDP; RDP; hRDP
• UniProt Entry Name: DPEP1_HUMAN

NCBI Description

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: EC 3.4.13.19; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space; apical part of cell; microvillus membrane; apical plasma membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; metalloexopeptidase activity; zinc ion binding; dipeptidyl-peptidase activity; caspase inhibitor activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process; arachidonic acid metabolic process; leukotriene metabolic process; homocysteine metabolic process; negative regulation of caspase activity; proteolysis; negative regulation of cell migration; antibiotic metabolic process; negative regulation of apoptosis