Nuclear Factor Kappa B2 (NFkB2) Recombinant Protein | NFkB2 recombinant protein

Recombinant Nuclear Factor Kappa B2 (NFkB2)

Cat. Num. AAA2009649

• Gene Names: Nfkb2; lyt; p49; p52; p50B; p49/p100; NF-kappaB2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Nuclear Factor Kappa B2 (NFkB2); Recombinant Nuclear Factor Kappa B2 (NFkB2); NFkB2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-ADGPYL VIVEQPKQRG FRFRYGCEGP SHGGLPGASS EKGRKTYPTV KICNYEGPAK IEVDLVTHSD PPRAHAHSLV GKQCSELGVC AVSVGPKDMT AQFNNLGVLH VTKKNMMEIM IQKLQRQRLR SKPQGLTEAE RRELEQEAKE LKKVMDLSIV RLRFSAFLRA SDGSFSLPLK PVISQPIHDS KSPGA
• Sequence Length: 899

• Applicable Applications for NFkB2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala35~Ala225 (Accession # Q9WTK5) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 293651546
• NCBI GeneID: 18034
• NCBI Accession #: NP_001170840.1
• NCBI GenBank Nucleotide #: NM_001177369.1
• UniProt Accession #: Q9WTK5
• Molecular Weight: 24.9kDa
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
• NCBI Official Symbol: Nfkb2
• NCBI Official Synonym Symbols: lyt; p49; p52; p50B; p49/p100; NF-kappaB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit; NF kappaB2; DNA-binding factor KBF2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: Nfkb2
• UniProt Entry Name: NFKB2_MOUSE

Uniprot Description

NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; follicular dendritic cell differentiation; cellular response to stress; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; lymph node development; regulation of transcription, DNA-dependent; response to cytokine stimulus; germinal center formation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response