Mouse Nuclear Factor Kappa B2 (NFkB2) ELISA Kit | NFkB2 elisa kit

Mouse Nuclear Factor Kappa B2 (NFkB2) ELISA Kit

Cat. Num. AAA452731

• Gene Names: Nfkb2; lyt; p49; p52; p50B; p49/p100; NF-kappaB2
• Reactivity: Mouse
• Synonyms: Nuclear Factor Kappa B2 (NFkB2); Mouse Nuclear Factor Kappa B2 (NFkB2) ELISA Kit; LYT-10; H2TF1; Lymphocyte translocation chromosome 10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2(p49/P100); Nuclear factor NF-kappa-B p52; NFkB2 elisa kit

• Reactivity: Mouse
• Sequence Length: 899

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra Assay: CV<10%
• Inter Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 293651546
• NCBI GeneID: 18034
• NCBI Accession #: NP_001170840.1
• NCBI GenBank Nucleotide #: NM_001177369.1
• UniProt Accession #: Q9WTK5
• Molecular Weight: 96,832 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
• NCBI Official Symbol: Nfkb2
• NCBI Official Synonym Symbols: lyt; p49; p52; p50B; p49/p100; NF-kappaB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: Nfkb2

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65 (). In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.