Testing Data
Mouse anti-Human CD155 Monoclonal Antibody | anti-CD155 antibody
Monoclonal anti-human CD155 (PVR)/R-PE
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
CD155; Monoclonal Antibody; Monoclonal anti-human CD155 (PVR)/R-PE; anti-CD155 (PVR; ANC2B2); anti-human CD155 (PVR); Poliovirus Recdeptor; PVR; Necl-5; anti-CD155 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
ANC2B2
Specificity
Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.
Antibodies ANC2B2 and ANC6A3 (#MBS666907) each bind to distict epitopes of CD155 and are suitable as a matched pair for sandwich EIA.
Antibodies ANC2B2 and ANC6A3 (#MBS666907) each bind to distict epitopes of CD155 and are suitable as a matched pair for sandwich EIA.
Form/Format
50mM Sodium Phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).
Concentration
Volume in Vial: 0.2 ml (varies by lot)
Applicable Applications for anti-CD155 antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Conjugation
R-PE
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrinthrough a sulfo-ester linkage. Unconjugated antibody wasremoved using size exclusion chromatography. Antibody conjugate is at 1.0mg/ml with an A565/A280 ratio of 3.04.
Performance
Five x 105 cultured human U-937 cells pertube were washed and preincubated 5 minutes with 20ul of 300ug/ml human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-human CD155/R-PE at a dilution factor of 1:50 (20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stainedpositive with a mean shift of 0.5 log10 fluorescent units whencompared to a Mouse IgG1/R-PE negative control (#MBS666704) at a similar concentration. Binding was blocked when cellswere preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD3antibody (#MBS666567).
Preparation and Storage
Store at 2-5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD155 antibody
Human CD155 (Polio Virus Receptor, PVR, Necl-5) is a 70kd type I Ig superfamily molecule (1).1 It is involved in formation of intracellular junctions between epithelial cells. Its ligands include CD226 (DNAM-1), and CD96 (TACTILE). CD155 expression by tumor has been shown to be upregulated by Nitric Oxide (2). A soluble version of CD155 has been shown to exist (4). High CD155 expression has recently been exploited to use engineered poliovirus to treat glioblastoma. (3)
References
1) Medelsohn CL, Racaniello VR, et al. (1989) Cell 56 (5): 855-65.
2) C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15 (1):17 PMID 25609078.
3) Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16 (supp3): iii41.
4) Baury B, MG Denis, et al. (2003) Biochem Biophys Res Comm 309: 175-82.
2) C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15 (1):17 PMID 25609078.
3) Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16 (supp3): iii41.
4) Baury B, MG Denis, et al. (2003) Biochem Biophys Res Comm 309: 175-82.
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Product Notes
The CD155 (Catalog #AAA666954) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal anti-human CD155 (PVR)/R-PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD155 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD155 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD155, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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