RDBP recombinant protein

RDBP, 1-380aa, Human, His tag, E Coli

Cat. Num. AAA203881

• Gene Names: NELFE; RD; RDP; RDBP; D6S45; NELF-E
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RDBP; 1-380aa; Human; His tag; E Coli; RD RNA binding protein; D6S45; NELF-E; RD; RDP; NELFe; RDBP recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF
• Sequence Length: 380

• Applicable Applications for RDBP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RDBP recombinant protein

RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Recombinant human RDBP protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RDBP recombinant protein

Transcription & Translation

References

Wada T., et al. (2000) Mol Cell. 5 (6):1067-72.

NCBI and Uniprot Product Information

• NCBI GI #: 14670268
• NCBI GeneID: 7936
• NCBI Accession #: NP_002895
• NCBI GenBank Nucleotide #: NM_002904.5
• UniProt Accession #: P18615; Q5JP74; Q5JP75; Q96F56; Q9NPK2; A2BE08; B4DUN1; B4DYX9
• Molecular Weight: 45.4 kDa (400aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: negative elongation factor E
• NCBI Official Synonym Full Names: negative elongation factor complex member E
• NCBI Official Symbol: NELFE
• NCBI Official Synonym Symbols: RD; RDP; RDBP; D6S45; NELF-E
• NCBI Protein Information: negative elongation factor E
• UniProt Protein Name: Negative elongation factor E
• UniProt Gene Name: NELFE
• UniProt Synonym Gene Names: RD; RDBP; NELF-E

NCBI Description

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.