TPPP3 recombinant protein

TPPP3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121675

• Gene Names: TPPP3; p20; CGI-38; TPPP/p20; p25gamma
• Purity: >85% as determined by SDS-PAGE
• Synonyms: TPPP3; TPPP3 Protein; Human; Recombinant (His Tag); Human TPPP3 Protein (His Tag); CGI-38 Protein; p20 Protein; p25gamma Protein; TPPP/p20 Protein; tubulin polymerization-promoting protein family member 3; TPPP3 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys176

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human TPPP3 (Q9BW30)(Met1-Lys176) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TPPP3 recombinant protein

Background: TPPP3, a member of the Tubulin polymerization-promoting protein family, is an intrinsically unstructured protein that induces tubulin polymerization. TPPP3 is a marker in the developing musculoskeletal system. In tendons, Tppp3 is expressed in cells at the circumference of the developing tendons, likely the progenitors of connective tissues that surround tendons: the tendon sheath, epitenon, and paratenon. Tppp3 is also expressed in forming synovial joints. The onset of Tppp3 expression in joints coincides with cavitation, representing a molecular marker that can be used to indicate this stage in joint transition in joint differentiation. In late embryonic stages, Tppp3 expression highlights other demarcation lines that surround differentiating tissues in the forelimb. Depletion of TPPP3 by microRNA-based RNA interference (RNAi) inhibits cell growth, arrests cell cycles, and causes mitotic abnormalities in HeLa cells. C57BL/6 mice that received subcutaneously injected LLC (Lewis lung carcinoma) cells in which TPPP3 was knocked down showed a pronounced reduction in tumor progression. The migration/invasion activity of TPPP3-knockdown LLC cells was significantly suppressed in a transwell chamber migration assay. When these cells were injected into the tail veins of C57BL/6 mice, they exhibited milder lung metastasis compared with control tumor cells. Taken together, these findings suggested that the TPPP3 gene played an important role in tumorigenesis and metastasis, and it could potentially become a novel target for cancer therapy.

References

Staverosky JA, et al. (2009) Tubulin polymerization-promoting protein family member 3, Tppp3, is a specific marker of the differentiating tendon sheath and synovial joints. Dev Dyn. 238(3):685-92.Zhou W, et al. (2010) Stable knockdown of TPPP3 by RNA interference in Lewis lung carcinoma cell inhibits tumor growth and metastasis. Mol Cell Biochem. 343(1-2):231-8.

NCBI and Uniprot Product Information

• NCBI GI #: 56676377
• NCBI GeneID: 51673
• NCBI Accession #: NP_057048.2
• NCBI GenBank Nucleotide #: NM_015964.3
• UniProt Accession #: Q9BW30; Q49AH9; Q9Y326; Q9Y6H0
• Molecular Weight: 18,985 Da
• NCBI Official Full Name: tubulin polymerization-promoting protein family member 3
• NCBI Official Synonym Full Names: tubulin polymerization promoting protein family member 3
• NCBI Official Symbol: TPPP3
• NCBI Official Synonym Symbols: p20; CGI-38; TPPP/p20; p25gamma
• NCBI Protein Information: tubulin polymerization-promoting protein family member 3
• UniProt Protein Name: Tubulin polymerization-promoting protein family member 3
• Protein Family: Tubulin polymerization-promoting protein family
• UniProt Gene Name: TPPP3

Uniprot Description

Binds tubulin and has microtubule bundling activity. May play a role in cell proliferation and mitosis.