NELFE recombinant protein

NELFE, 1-380aa, Human, His tag, E Coli

Cat. Num. AAA205569

• Gene Names: NELFE; RD; RDP; RDBP; D6S45; NELF-E
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: NELFE; 1-380aa; Human; His tag; E Coli; Negative elongation factor E; Negative elongation factor complex member E; D6S45; NELF-E; RD; RDBP; RDP; NELFE recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF
• Sequence Length: 380

• Applicable Applications for NELFE recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NELFE recombinant protein

NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for NELFE recombinant protein

Epigenomics (Transcription & Translation)

References

Yamaguchi Y., Takagi T, et al. (1999) Cell 97:41-51; Surowy C.S., Hoganson G, et al. (1990) Gene 90:299-302;

NCBI and Uniprot Product Information

• NCBI GI #: 14670268
• NCBI GeneID: 7936
• NCBI Accession #: NP_002895
• NCBI GenBank Nucleotide #: NM_002904.5
• UniProt Accession #: P18615; Q5JP74; Q5JP75; Q96F56; Q9NPK2; A2BE08; B4DUN1; B4DYX9
• Molecular Weight: 45.6kDa (403aa) confirmed by MALDI-TOF
• NCBI Official Full Name: negative elongation factor E
• NCBI Official Synonym Full Names: negative elongation factor complex member E
• NCBI Official Symbol: NELFE
• NCBI Official Synonym Symbols: RD; RDP; RDBP; D6S45; NELF-E
• NCBI Protein Information: negative elongation factor E
• UniProt Protein Name: Negative elongation factor E
• UniProt Gene Name: NELFE
• UniProt Synonym Gene Names: RD; RDBP; NELF-E

NCBI Description

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.