NCBP2 recombinant protein

NCBP2, 1-156aa, Human, His tag, E Coli

Cat. Num. AAA203966

• Gene Names: NCBP2; CBC2; NIP1; CBP20; PIG55
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NCBP2; 1-156aa; Human; His tag; E Coli; Nuclear cap-binding protein subunit 2; CBC2; CBP20; NIP1; PIG55; NCBP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ
• Sequence Length: 156

• Applicable Applications for NCBP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2 degreee C to 8 degree C for 1 week.; For long term storage, aliquot and store at -20 degree C to -80 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for NCBP2 recombinant protein

NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Recombinant human NCBP2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NCBP2 recombinant protein

Transcription & Translation

References

Izaurralde E., et al. (1994) Cell. 78: 657-668.; Strasser A., et al. ( (2005) EMBO J 24 (13):2235-43.

NCBI and Uniprot Product Information

• NCBI GI #: 19923387
• NCBI GeneID: 22916
• NCBI Accession #: NP_031388
• NCBI GenBank Nucleotide #: NM_007362.3
• UniProt Accession #: P52298; Q14924; Q2TS50; B2RE91; B4DMK7; E9PAR5
• Molecular Weight: 20.1 kDa (176aa), confirmed by MALDI-TOF
• NCBI Official Full Name: nuclear cap-binding protein subunit 2 isoform 1
• NCBI Official Synonym Full Names: nuclear cap binding protein subunit 2
• NCBI Official Symbol: NCBP2
• NCBI Official Synonym Symbols: CBC2; NIP1; CBP20; PIG55
• NCBI Protein Information: nuclear cap-binding protein subunit 2
• UniProt Protein Name: Nuclear cap-binding protein subunit 2
• Protein Family: Nuclear cap-binding protein
• UniProt Gene Name: NCBP2
• UniProt Synonym Gene Names: CBP20; CBP20; NIP1

NCBI Description

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).