Mouse Ornithine aminotransferase Antibody | anti-OAT antibody

Mouse Anti Human Ornithine aminotransferase

Cat. Num. AAA140358

• Gene Names: OAT; OKT; GACR; HOGA; OATASE
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: OAT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Ornithine aminotransferase; Mouse Anti Human Ornithine aminotransferase; OAT Antibody; Mouse Anti Human; Ornithine aminotransferase mitochondrial; Ornithine delta-aminotransferase; Ornithine-oxo-acid aminotransferase; OAT; OKT; GACR; HOGA; OATASE; DKFZp781A11155; anti-OAT antibody

• Host: Mouse
• Isotype: IgG1 heavy chain and kappa light chain
• Purity/Purification: OAT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 439

• Applicable Applications for anti-OAT antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: OAT antibody has been tested by by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.Recommended starting dilution is 1:250.
• Immunogen: Anti-human OAT mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human OAT amino acids 33-439 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-OAT antibody

Introduction: Ornithine aminotransferase is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Ornithine aminotransferase (OAT) is a 49kDa nucleus-encoded protein imported into mitochondria to give the mature 48kDa OAT polypeptide. It is found in humans, animals, insects, plants and microorganisms. The OAT has a sex-differential expression in the mouse kidney. OAT plays central physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases that transfer an amino group from a carbon atom which doesn't carry a carboxyl function. OAT is vital for nitrogen recycling from arginine but not for the stress-induced proline accumulation. OAT enzyme deficiency causes the autosomal recessive eye disease Gyrate Atrophy.

Product Categories/Family for anti-OAT antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 4557809
• NCBI GeneID: 4942
• NCBI Accession #: NP_000265.1
• NCBI GenBank Nucleotide #: NM_000274.3
• Molecular Weight: 32,853 Da
• NCBI Official Full Name: ornithine aminotransferase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: ornithine aminotransferase
• NCBI Official Symbol: OAT
• NCBI Official Synonym Symbols: OKT; GACR; HOGA; OATASE
• NCBI Protein Information: ornithine aminotransferase, mitochondrial; gyrate atrophy; ornithine delta-aminotransferase; ornithine-oxo-acid aminotransferase
• UniProt Protein Name: Ornithine aminotransferase, mitochondrial
• Protein Family: Ornithine aminotransferase
• UniProt Gene Name: OAT
• UniProt Entry Name: OAT_HUMAN

NCBI Description

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]

Uniprot Description

OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.6.1.13; Amino Acid Metabolism - arginine and proline; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding

Biological Process: visual perception; amino acid biosynthetic process

Disease: Gyrate Atrophy Of Choroid And Retina