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Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human lung with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human Peroxiredoxin 5 Polyclonal Antibody | anti-PRDX5 antibody
Anti-Peroxiredoxin 5 Antibody, Rabbit Polyclonal
Gene Names
PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Peroxiredoxin 5; Polyclonal Antibody; Anti-Peroxiredoxin 5 Antibody; Rabbit Polyclonal; Peroxiredoxin 5/PRDX5 Antibody; Rabbit PAb; Antigen Affinity Purified; peroxiredoxin 5; Anti-ACR1 Antibody; Anti-AOEB166 Antibody; Anti-B166 Antibody; Anti-HEL-S-55 Antibody; Anti-PLP Antibody; Anti-PMP20 Antibody; Anti-PRDX6 Antibody; Anti-prx-V Antibody; Anti-PRXV Antibody; Anti-SBBI10 Antibody; anti-PRDX5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Peroxiredoxin 5/PRDX5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRDX5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
Immunogen
Recombinant Human Peroxiredoxin 5/PRDX5 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Peroxiredoxin 5/PRDX5 (rh Peroxiredoxin 5/PRDX5). Peroxiredoxin 5/PRDX5 specific IgG was purified by Human Peroxiredoxin 5/PRDX5 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human lung with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human lung with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PRDX5 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunoprecipitation (IP)
(PRDX5 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PRDX5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX5 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Immunoprecipitation (IP)
(PRDX5 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PRDX5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX5 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Western Blot (WB)
(Anti-PRDX5 rabbit polyclonal antibody at 1:2000 dilutionLane A: Hela Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:15 kDa)
Western Blot (WB)
(Anti-PRDX5 rabbit polyclonal antibody at 1:2000 dilutionLane A: Hela Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:15 kDa)
Related Product Information for anti-PRDX5 antibody
Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein that belongs to the peroxiredoxin 2 family. Peroxiredoxin-5/PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5/PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins/Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins/Prx family is divided into six groups (PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins/Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins/Prx has a proliferative effect and may be related to cancer development or progression.
References
Seo M.S., et al., 2000, J. Biol. Chem. 275: 20346-54. Declercq J.-P., et al., 2001, J. Mol. Biol. 311:751-9. Noh, D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Schremmer, B. et al., 2007, Subcell Biochem. 44 :317-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
214
NCBI Official Full Name
peroxiredoxin-5, mitochondrial isoform a
NCBI Official Synonym Full Names
peroxiredoxin 5
NCBI Official Symbol
PRDX5
NCBI Official Synonym Symbols
PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166
NCBI Protein Information
peroxiredoxin-5, mitochondrial; TPx type VI; peroxiredoxin V; Alu co-repressor 1; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; liver tissue 2D-page spot 71B; peroxisomal antioxidant enzyme
UniProt Protein Name
Peroxiredoxin-5, mitochondrial
Protein Family
UniProt Gene Name
PRDX5
UniProt Synonym Gene Names
ACR1; AOEB166; Prx-V
UniProt Entry Name
PRDX5_HUMAN
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Product Notes
The PRDX5 prdx5 (Catalog #AAA8105174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peroxiredoxin 5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:1000-1:5000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the PRDX5 prdx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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