Rabbit anti-Human, Mouse MED21 Polyclonal Antibody | anti-MED21 antibody

Rabbit Polyclonal (IgG) to Human MED21

Cat. Num. AAA244641

• Gene Names: MED21; SRB7; SURB7; hSrb7
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MED21; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human MED21; Anti-MED21 Antibody (aa71-120) IHC-plus; HSrb7; RNAPII complex component SRB7; SRB7; SURB7; Mediator complex subunit 21; Human MED21; anti-MED21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED21 Antibody detects endogenous levels of total MED21 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 144

• Applicable Applications for anti-MED21 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MED21 (aa71-120).
• Immunogen: MED21 antibody was raised against synthetic peptide from human MED21 (aa71-120).
• Antigen Modification: aa71-120
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-MED21 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361161
• NCBI GeneID: 9412
• NCBI Accession #: NP_004255.2
• NCBI GenBank Nucleotide #: NM_004264.4
• UniProt Accession #: Q13503; Q6IB05; Q92811; B2R4I3
• Molecular Weight: 15,564 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 21 isoform 1
• NCBI Official Synonym Full Names: mediator complex subunit 21
• NCBI Official Symbol: MED21
• NCBI Official Synonym Symbols: SRB7; SURB7; hSrb7
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 21; RNAPII complex component SRB7; RNA polymerase II holoenzyme component SRB7; SRB7 suppressor of RNA polymerase B homolog
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 21
• Protein Family: Putative mediator of RNA polymerase II transcription
• UniProt Gene Name: MED21
• UniProt Synonym Gene Names: SRB7; SURB7; RNAPII complex component SRB7; hSrb7
• UniProt Entry Name: MED21_HUMAN

NCBI Description

This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

MED21: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 21 family.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p11.23

Cellular Component: Srb-mediator complex

Molecular Function: protein binding; DNA-directed RNA polymerase activity; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; blastocyst development; transcription, DNA-dependent; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter