Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) Standard Curve.)
Human Non-Receptor Tyrosine-Protein Kinase TNK1 (TNK1) ELISA Kit | TNK1 elisa kit
Human Non-Receptor Tyrosine-Protein Kinase TNK1 (TNK1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) Standard Curve.)
Background: TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC and JAK families, mediate intracellular signaling downstream of receptor activation. TNK1 contains a putative N-terminal phosphotyrosine-binding domain, followed by the tyrosine kinase domain, a putative SH3 domain, and a proline-rich C terminus that contains SH3-binding motifs. The kinase domain has all 12 structural features conserved in tyrosine kinases, including an ATP-binding site. TNK1 shares highest homology with ACK, including 45% amino acid identity over a 413-amino acid span that includes the kinase domain and putative SH3 domain. specific expression of 4 transcripts between 1.0 and 9.5 kb in fetal brain, lung, liver, and kidney.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
TNK1: Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction. Interacts with the SH3 domain of PLCG1 via its Pro-rich domain. Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain. Belongs to the protein kinase superfamily. Tyr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Ack family
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: extrinsic to internal side of plasma membrane; membrane; cytoplasm; cytosol
Molecular Function: protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; hormone receptor binding
Biological Process: cell migration; protein amino acid autophosphorylation; negative regulation of Ras protein signal transduction; innate immune response; negative regulation of cell growth; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Research Articles on TNK1
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Product Notes
The Human TNK1 tnk1 (Catalog #AAA9713864) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713864 ELISA Kit recognizes Human TNK1. It is sometimes possible for the material contained within the vial of "Non-Receptor Tyrosine-Protein Kinase TNK1 (TNK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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