Mouse Prealbumin (PA) ELISA Kit | PA elisa kit

Mouse Prealbumin (PA) ELISA Kit

Cat. Num. AAA9717401

• Gene Names: LCN1; TP; TLC; PMFA; VEGP
• Reactivity: Mouse
• Synonyms: Prealbumin (PA); Mouse Prealbumin (PA) ELISA Kit; PA elisa kit

• Reactivity: Mouse
• Specificity: Mouse Prealbumin (PA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PA. No significant cross-reactivity or interference between Mouse PA and analogues was observed.
• Sequence Length: 176

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Prealbumin (PA) Standard Curve.)

Related Product Information for PA elisa kit

Description: This Mouse Prealbumin (PA) ELISA Kit employs a two-site sandwich ELISA to quantitate PA in samples. An antibody specific for PA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TTR was originally called prealbumin because it ran faster than albumins on electrophoresis gels.Prealbumin is produced by the choroid plexus, by pancreatic islet cells in the embryonic yolk sac, and by enterochromaffin cells in the gastrointestinal mucosa, but the liver is quantitatively the most important source.9 Liver production is maintained until late in liver disease. Hydration status does not affect prealbumin levels. A negative acute phase reactant, the prealbumin level will transiently decrease in the presence of inflammation and in the immediate postsurgical period. Serum levels also decline in patients with conditions associated with protein malnutrition, such as malignancy, cirrhosis, protein-losing enteropathy, and zinc deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 184458
• NCBI GeneID: 3933
• NCBI Official Full Name: prealbumin
• NCBI Official Synonym Full Names: lipocalin 1
• NCBI Official Symbol: LCN1
• NCBI Official Synonym Symbols: TP; TLC; PMFA; VEGP
• NCBI Protein Information: lipocalin-1
• Protein Family: Protein

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]