Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit | MIP1a elisa kit

Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit

Cat. Num. AAA2701147

• Reactivity: Mouse
• Synonyms: Macrophage Inflammatory Protein 1 Alpha (MIP1a); Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit; CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein; MIP1a elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).; No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
• Sequence Length: 70

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP1a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Alpha (MIP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2905628
• UniProt Accession #: P10147
• Molecular Weight: 10,085 Da
• NCBI Official Full Name: macrophage inflammatory protein 1 alpha, partial
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: B-box domain protein
• UniProt Gene Name: CCL3
• UniProt Synonym Gene Names: G0S19-1; MIP1A; SCYA3; MIP-1-alpha

Uniprot Description

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).