Human Lipocalin-1 ELISA Kit | LCN1 elisa kit

Human Lipocalin-1 ELISA Kit

Cat. Num. AAA761271

• Gene Names: LCN1; TP; TLC; PMFA; VEGP
• Reactivity: Human
• Synonyms: Lipocalin-1; Human Lipocalin-1 ELISA Kit; LCN1/Lipocalin-1/PMFA/TLC/TP/VEGP/Von Ebner gland protein/VEG protein/Tear lipocalin/Tear prealbumin; LCN1 elisa kit

• Reactivity: Human
• Sequence Length: 230

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LCN1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LCN1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LCN1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LCN1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LCN1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 357933621
• NCBI GeneID: 3933
• NCBI Accession #: NP_001239548.1
• NCBI GenBank Nucleotide #: NM_001252619.1
• Molecular Weight: 19,250 Da
• NCBI Official Full Name: lipocalin-1 isoform 3
• NCBI Official Synonym Full Names: lipocalin 1
• NCBI Official Symbol: LCN1
• NCBI Official Synonym Symbols: TP; TLC; PMFA; VEGP
• NCBI Protein Information: lipocalin-1
• UniProt Protein Name: Lipocalin-1
• Protein Family: Lipocalin
• UniProt Gene Name: LCN1
• UniProt Synonym Gene Names: VEGP; Tlc; TP; VEG protein
• UniProt Entry Name: LCN1_HUMAN

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular region; extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: long-chain fatty acid transport; proteolysis; retinal homeostasis