Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: K562, MCF7 and hepg2 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds)
Rabbit anti-Human ACSS2 Polyclonal Antibody | anti-ACSS2 antibody
ACSS2 Antibody
Gene Names
ACSS2; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
ACSS2; Polyclonal Antibody; ACSS2 Antibody; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1; anti-ACSS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ACSS2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
714
Applicable Applications for anti-ACSS2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:50-1:200
Immunohistochemistry: 1:50-1:200
Immunogen
Fusion protein of human ACSS2
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: K562, MCF7 and hepg2 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-3: K562, MCF7 and hepg2 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Related Product Information for anti-ACSS2 antibody
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-ACSS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,087 Da
NCBI Official Full Name
acetyl-coenzyme A synthetase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
acyl-CoA synthetase short-chain family member 2
NCBI Official Symbol
ACSS2
NCBI Official Synonym Symbols
ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
NCBI Protein Information
acetyl-coenzyme A synthetase, cytoplasmic
UniProt Protein Name
Acetyl-coenzyme A synthetase, cytoplasmic
Protein Family
UniProt Gene Name
ACSS2
UniProt Synonym Gene Names
ACAS2; ACS; AceCS
UniProt Entry Name
ACSA_HUMAN
Uniprot Description
ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 6.2.1.1; Ligase
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm
Molecular Function: acetate-CoA ligase activity; AMP binding
Biological Process: ethanol oxidation; lipid biosynthetic process
Similar Products
Product Notes
The ACSS2 acss2 (Catalog #AAA9415747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACSS2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the ACSS2 acss2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
