Human Acyl CoA dehydrogenase family member 11 (ACAD11) ELISA Kit | ACAD11 elisa kit

Human Acyl CoA dehydrogenase family member 11 (ACAD11) ELISA Kit

Cat. Num. AAA7249370

• Gene Names: ACAD11; ACAD-11
• Reactivity: Human
• Synonyms: Acyl CoA dehydrogenase family member 11 (ACAD11); Human Acyl CoA dehydrogenase family member 11 (ACAD11) ELISA Kit; ACAD11 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACAD11 elisa kit

Intended Uses: This ACACB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACACB2. This ELISA kit for research use only!

Principle of the Assay: ACACB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACACB2 antibody and an ACACB2-HRP conjugate. The assay sample and buffer are incubated together with ACACB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACACB2 concentration since ACACB2 from samples and ACACB2-HRP conjugate compete for the anti-ACACB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACACB2 from the sample, fewer sites are left to bind ACACB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACACB2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38505218
• NCBI GeneID: 84129
• NCBI Accession #: NP_115545.3
• NCBI GenBank Nucleotide #: NM_032169.4
• Molecular Weight: 73,920 Da
• NCBI Official Full Name: acyl-CoA dehydrogenase family member 11
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase family, member 11
• NCBI Official Symbol: ACAD11
• NCBI Official Synonym Symbols: ACAD-11
• NCBI Protein Information: acyl-CoA dehydrogenase family member 11; 62113 protein; acyl-Coenzyme A dehydrogenase family, member 11
• UniProt Protein Name: Acyl-CoA dehydrogenase family member 11
• UniProt Gene Name: ACAD11
• UniProt Synonym Gene Names: ACAD-11
• UniProt Entry Name: ACD11_HUMAN

NCBI Description

This gene encodes an acyl-CoA dehydrogenase enzyme with a preference for carbon chain lengths between 20 and 26. Naturally occurring read-through transcription occurs between the upstream gene NPHP3 (nephronophthisis 3 (adolescent)) and this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

ACAD11: Acyl-CoA dehydrogenase, that exhibits maximal activity towards saturated C22-CoA. Belongs to the acyl-CoA dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.3.99.-

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: mitochondrial membrane; peroxisome; nucleus

Molecular Function: FAD binding; transferase activity, transferring phosphorus-containing groups; very-long-chain-acyl-CoA dehydrogenase activity; long-chain-acyl-CoA dehydrogenase activity

Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase